Part:BBa_I725007:Design

butyraldehyde/butanol DH 2

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 169
  Illegal AgeI site found at 571
  Illegal AgeI site found at 1795
  Illegal AgeI site found at 1840
  Illegal AgeI site found at 1942
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This sequence has been optimized for E. coli and has had its restriction sites removed.

Source

C. acetobutylicum pSOL plasmid